Métodos para detecção de Ranavirus (Iridoviridae) em anfíbios
DOI:
https://doi.org/10.5281/zenodo.5211381Resumo
Dentre os muitos fatores que impactam negativamente a biodiversidade mundial (como desmatamento, poluição e aquecimento global), as doenças infecciosas são consideradas um grande problema para a conservação da flora e fauna. Principalmente em relação aos anfíbios, que constituem o grupo de vertebrados mais ameaçado da atualidade, as doenças infecciosas já foram responsáveis por declínios e extinções de populações naturais em todos os continentes. Os principais patógenos conhecidos até o momento que apresentam maior impacto sobre esse grupo são os dois fungos causadores da quitridiomicose (Batrachochytrium dendrobatidis e Batrachochytrium salamandrivorans) e os vírus do gênero Ranavirus. Os vírus do gênero Ranavirus constituem uma ameaça não somente aos anfíbios, mas também aos peixes e répteis, e já foram relacionados a eventos de mortalidade em massa de indivíduos desses grupos em diversos países. A recente descoberta desse vírus em populações naturais de anfíbios no Brasil despertou o nosso interesse em apresentar uma revisão de protocolos para a detecção do Ranavirus a fim de incentivar pesquisadores a realizar diagnósticos de ranavirose em vertebrados ectotérmicos, em especial anfíbios.Referências
Allender M.C., Bunick D., Mitchell M.A. 2013. Development and validation of TaqMan quantitative PCR for detection of frog virus 3-like virus in eastern box turtles (Terrapene carolina carolina). Journal of Virological Methods 188:121–125.
Brunner J.L., Olson A.D., Rice J.G., Meiners S.E., Le Sage M.J., Cundiff J.A., ... Pessier A.P. 2019. Ranavirus infection dynamics and shedding in American bullfrogs: consequences for spread and detection in trade. Diseases of Aquatic Organisms 135:135–150.
Bryan L.K., Baldwin C.A., Gray M.J., Miller D.L. 2009. Efficacy of select disinfectants at inactivating Ranavirus. Diseases of Aquatic Organisms 84:89–94.
Candido M., Sousa R.L.M. 2018. Aspectos celulares e moleculares da ranavirose experimental em tilápias do Nilo (Oreochromis niloticus). Ph.D. Dissertation, Universidade de São Paulo, Brasil.
Candido M., Tavares L.S., Alencar A.L.F., Ferreira C.M., Queiroz S.R.A., Fernandes A.M., Sousa R.L.M. 2019. Genome analysis of Ranavirus frog virus 3 isolated from American Bullfrog (Lithobates catesbeianus) in South America. Scientific Reports 9:17135.
Chinchar G.V. 2002. Ranaviruses (family Iridoviridae): emerging cold-blooded killers. Archives of Virology 147:447–470.
Dimitrieva S., Bucher P. 2013. UCNEbase – a database of ultraconserved non-coding elements and genomic regulatory blocks. Nucleic Acids Research 41(D1):D101–D109.
Duffus A.L.J., Waltzek T.B., Stöhr A.C., Allender M.C., Gotesman M., Whittington R.J., ... Marschang R.E. 2015. Distribution and host range of ranaviruses. Pp. 9–57 in Gray M.J., Chinchar V.G. (Eds), Ranaviruses: lethal pathogens of ectothermic vertebrates. Springer, New York.
Forzán M.J., Wood J. 2013. Low detection of ranavirus DNA in wild postmetamorphic green frogs, Rana (Lithobates) clamitans, despite previous or concurrent tadpole mortality. Journal of Wildlife Diseases 49:879–886.
Galli L., Pereira A., Márquez A., Mazzoni R. 2006. Ranavirus detection by PCR in cultured tadpoles (Rana catesbeiana Shaw, 1802) from South America. Aquaculture 257:78–82.
Garland S., Backer A., Phillott A.D., Skerratt L.F. 2010. BSA reduces inhibition in a TaqMan® assay for the detection of Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms 92:113–116.
Goodman R.M., Miller D.L., Ararso Y.T. 2013. Prevalence of ranavirus in Virginia turtles as detected by tail-clip sampling versus oral-cloacal swabbing. Northeastern Naturalist 20:325–332.
Gray M.J., Miller D.L., Hoverman J.T. 2012. Reliability of non-lethal surveillance methods for detecting ranavirus infection. Diseases of Aquatic Organisms 99:1–6.
Greer A.L., Berrill M., Wilson P.J. 2005. Five amphibian mortality events associated with ranavirus infection in south central Ontario, Canada. Diseases of Aquatic Organisms 67:9–14.
Jancovich J.K., Qin Q., Zhang V.G. 2015. Ranavirus replication: molecular, cellular, and immunological events. Pp. 105–139 in Gray M.J., Chinchar V.G. (Eds), Ranaviruses: lethal pathogens of ectothermic vertebrates. Springer, New York.
Jaramillo D., Tweedie A., Becker J.A., Hyatt A., Crameri S., Whittington R.J. 2012. A validated quantitative polymerase chain reaction assay for the detection of ranaviruses (Family Iridoviridae) in fish tissue and cell cultures, using EHNV as a model. Aquaculture 356:186–192.
Julian J.T., Glenney G.W., Rees C. 2019. Evaluating observer bias and seasonal detection rates in amphibian pathogen eDNA collections by citizen scientists. Diseases of Aquatic Organisms 134:15–24.
Kolby J.E., Smith K.M., Berger L., Karesh W.B., Preston A., Pessier A.P., Skerratt L.F. 2014. First Evidence of Amphibian Chytrid Fungus (Batrachochytrium dendrobatidis) and Ranavirus in Hong Kong Amphibian Trade. PLoS One 9:e90750.
Leung W.T., Thomas-Walters L., Garner T.W., Balloux F., Durrant C., Price S.T. 2017. A quantitative-PCR based method to estimate Ranavirus viral load following normalization by reference to an ultraconserved vertebrate target. Journal of Virological Methods 249:147–155.
Mao J., Hedrick R.P., Chinchar V.G. 1997. Molecular characterization, sequence analysis, and taxonomic position of newly isolated fish iridoviruses. Virology 229:212–220.
MAPA – Ministério da Agricultura, Pecuária e Abastecimento. 2020. Electronic Database acessível em http://www.defesaagropecuaria.al.gov.br/sanidade-animal/FORM%20NOTIFICA%20%20Instrutivo.pdf.
Mazzoni R., Mesquita A.J., Fleury L.F.F., Brito W.M.E.D., Nunes I.A., Robert J., ... Catuzo M.H.B. 2009. Mass mortality associated with a virus 3-like Ranavirus infection in farmed tadpoles Rana catesbeiana from Brazil. Diseases of Aquatic Organisms 86:181–191.
Miaud C., Arnal V., Poulain M., Valentini A., Dejean T. 2019. eDNA increases the detectability of ranavirus infection in an alpine amphibian population. Viruses 11:526.
Miller D.L., Pessier A.P., Hick P., Whittington R.J. 2015. Comparative pathology of ranaviruses and diagnostic techniques. Pp. 171–208 in Gray M.J., Chinchar V.G. (Eds), Ranaviruses: lethal pathogens of ectothermic vertebrates. Springer, New York.
Pallister J., Gould A., Harrison D., Hyatt A., Jancovich J., Heine H. 2007. Development of real-time PCR assays for the detection and differentiation of Australian and European ranaviruses. Journal of Fish Diseases 30:427–438.
Pessier A.P., Mendelson J.R. 2010. A manual for control of infectious diseases in amphibian survival assurance colonies and reintroduction programs. IUCN/SSC Captive Breeding Specialist Group, Apple Valley, MN.
Price S.J., Garner T.W.J., Cunningham A.A., Langton T.E.S., Nichols R.A. 2016. Reconstructing the emergence of a lethal infectious disease of wildlife supports a key role for spread through translocations by humans. Proceedings of the Royal Society B 283:20160952.
Ruggeri J., Ribeiro L.P., Pontes M.R., Toffolo C., Candido M., Carreiro M.M., ... Toledo L.F. 2019. Discovery of wild amphibians infected with Ranavirus in Brazil. Journal of Wildlife Diseases 55:897–902.
Schloegel L.M., Picco A.M., Kilpatrick A.M., Davies A.J., Hyatt A.D., Daszak P. 2009. Magnitude of the US trade in amphibians and presence of Batrachochytrium dendrobatidis and Ranavirus infection in imported North American Bullfrogs (Rana catesbeiana). Biological Conservation 142:1420–1426.
Shaw G. 1802. General Zoology or Systematic Natural History (Volume III, Part 1). Amphibia, Thomas Davison, London.
Vilaça S.T., Grant S.A., Beaty L., Brunetti C.R., Congram M., Murray D.L., Wilson C.C., Kyle C.J. 2020. Detection of spatiotemporal variation in ranavirus distribution using eDNA. Environmental DNA 2:210–220.
von Essen M., Leung W.T.M., Bosch J., Pooley S., Ayres C., Price S.J. 2020. High pathogen prevalence in amphibian and reptile assemblage at a site with risk factors for dispersal in Galicia, Spain. PLoS One 15:e0236803.
Warne R.W., LaBumbard B., LaGrange S., Vredenburg V.T., Catenazzi A. 2016. Co-infection by chytrid fungus and Ranaviruses in wild and harvested frogs in the tropical Andes. PLoS One 11:e0145864.
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