Detecção do fungo Batrachochytrium dendrobatidis por meio de nested PCR

Autores

  • Luisa P. Ribeiro Autor
  • Silvio C. Almeida Autor
  • Jacqueline Kasue Kurissio Autor
  • João Pessoa Araújo Júnior Autor
  • Tarsila Franckin Autor
  • Luís Felipe Toledo Autor
  • Sandra M. G. Bosco Autor

Referências

Annis, S. L., Dastoor, F. P. J., Ziel, H., Daszak, P. e Longcore, J. E. 2004. A DNA-based assay identifies Batrachochytrium dendrobatidis in amphibian populations. Journal of Wildlife Diseases, 40: 420–428.

Becker, C. G., Rodriguez, D., Lambertini, C., Toledo, L. F. e Haddad, C. F. B. 2015. History of Batrachochytrium dendrobatidis in Amazonian Ectotherms. Ecology, 96: 1109–1121.

Berger, L., Speare, R., Daszak, P., Green, D. E., Cunningham, A. A., Goggin, C. L., Slocombe, R., Ragan, M. A., Hyatt, A. D., McDonald, K. R., Hines, H. B., Lips, K. R., Marantelli, G. e Parkes, H. 1998. Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proceedings of the National Academy of Sciences of the United States of America, 95: 9031–9036.

Boyle, D. G., Boyle, D. B., Olsen, V., Morgan, J. A. T. e Hyatt, A. D. 2004. Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Diseases of Aquatic Organisms, 60: 141–148.

Coutinho, S. D., Burke, J. C., Paula, C. D. e Rodrigues, M. T. e Catão-Dias, J. L. 2015. The use of singleplex and nested PCR to detect Batrachochytrium dendrobatidis in free-living frogs. Brazilian Journal of Microbiology, 46(2): 551–555.

De Paula, C. D. e Catão-Dias, J. L. 2011. Chytridiomycosis: a devastating emerging fungal. Brazilian Journal of Veterinary Pathology, 4(3): 250–258.

Goka, K., Yokoyama, J., Une, Y., Kuroki, T., Suzuki, K., Nakahara, M., Kobayashi, A., Inaba, S., Mizutani, T. e Oshima, Y. 2009. Amphibian chytridiomycosis in Japan: distribution, haplotypes and possible route of entry into Japan. Molecular Ecology, 18: 4757–4774.

Hyatt, A. D., Boyle, D. G., Olsen, V., Boyle, D. B., Berger, L., Obendorf, D., Dalton, A., Kriger, K., Hero, M., Hines, H., Phillott, R., Campbell, R., Marantelli, G., Gleason, F. e Colling, A. 2007. Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis. Diseases of Aquatic Organisms, 73: 175–192.

James, T. Y., Toledo, L. F., Rödder, D., Silva, A. A., Betancourt-Román, C. M., Jenkins, E. E., Soto-Azat, C., Lambertini, C., Longo, A. V., Ruggeri, J., Collins, J. P., Burrowes, P. A., Lips, K. R., Zamudio, K. R. e Longcore, J. E. 2015. Disentangling host, pathogen and environment determinants of a recently emerged wildlife disease: lessons from the first 15 years of amphibian chytridiomycosis research. Ecology and Evolution, 5(18): 4079–4097.

Lambertini, C., Rodriguez, D., Brito, F. B., Leite, D. S. e Toledo, L. F. 2013. Diagnóstico do fungo Quitrídio: Batrachochytrium dendrobatidis. Herpetologia Brasileira, 2: 127–137.

Lambertini, C., Becker, C. G., Jenkins, T. S., Rödder, D., Leite, D. S., James, T. Y., Zamudio, K. R. e Toledo, L. F. 2016. Local phenotypic variation in amphibian killing fungus predicts infection dynamics. Fungal Ecology, 20: 51–61.

Longcore, J. E., Pessier, A. P. e Nichols, D. K. 1999. Batrachochytrium dendrobatidis gen. et sp. nov., a chytrid pathogenic to amphibians. Mycologia, 91: 219–227.

McCullough, M. J., Disalvo, A. F., Clemons, K. V., Park, E. e Stevens, D. A. 2000. Molecular epidemiology of Blastomyces dermatitidis. Clinical Infectious Diseases, 30: 328–335.

Olson, D. H. e Ronnemberg, K. J. L. 2014. Global Bd mapping project: 2014 update. FrogLog, 111: 17–21.

Pessier, A. P. e Mendelson, J. R. 2009. A Manual for Control of Infectious Diseases in Amphibian Survival Assurance Colonies and Reintroduction Programs. Conservation Breeding Specialist Group, IUCN/SSC.

Rodriguez, D., Becker, C. G., Pippin, N. C., Haddad, C. F. B. e Zamudio, K. R. 2014. Long-term endemism of two highly divergent lineages of the amphibian-killing fungus in the Atlantic Forest of Brazil. Molecular Ecology, 23: 774–787.

Vieira, C. A. e Toledo, L. F. 2012. Isolamento, cultivo e armazenamento do fungo quitrídio: Batrachochytrium dendrobatidis. Herpetologia Brasileira, 1: 18–19.

White, T. J., Bruns, T., Lee, S. e Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1): 315–322.

Publicado

2026-05-06

Como Citar

RIBEIRO, Luisa P.; ALMEIDA, Silvio C.; KASUE KURISSIO, Jacqueline; PESSOA ARAÚJO JÚNIOR, João; FRANCKIN, Tarsila; TOLEDO, Luís Felipe; BOSCO, Sandra M. G. Detecção do fungo Batrachochytrium dendrobatidis por meio de nested PCR. Herpetologia Brasileira, [S. l.], v. 6, n. 1, p. 11–14, 2026. Disponível em: https://hb.sbherpetologia.org.br/index.php/hb/article/view/75. Acesso em: 15 jun. 2026.